Team:Paris Liliane Bettencourt/Notebook/2011/08/02/

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Adrien Lhomme-Duchadeuil

First, an estimation of how much antibiotics is necessary for Bacillus subtilis. Appropriate concentration in antibiotics

  • Chloramphemicol : 5-10 µg/mL
  • Spec : 50-100 µg/mL
  • Tetracyclin: 25 µg/mL
  • Kanamycin: 5µg/mL
  • Evm(?): 1µg/mL + 25 µg/mL lincomycin (Verify spelling)

Results of electroporation: IT WORKED after three trials!

  • Positive control worked
  • Negative controls didn't show any colonies except when put in the presence of TetR (qty:12,5 µg/mL) which was insufficient => I will re-plate the improbable survivors on plates of different tetracyclin concentrations to test resistance.
  • Cm resistant strain have survived in low quatities at both 30 and 5 µg/mL of antibiotics
  • It seems that even cuvettes that had an electric arc were able to produce a couple colonies, which means that it doesn't all the bacteria.
  • To come: protocol and table of results