Team:UC Davis/Notebook

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Contents

Week 7

--Monday 7/25/11--

We continued with construction today and, once again, extracted the cut Promoters+GFP and repressors from a gel. We ligated the promoters+GFP in from of I13453 and the repressors before B0034. Hopefully this is the last time we will have to ligate and transform these parts.

--Tuesday 7/26/11--

The insert controls for yesterday's transformations looked good, but there were still a good amount of colonies on the vector controls. We decided to do a PCR screen of these parts to make sure that they were the right length.

Today was also "Tip Day"! We filled enough tip boxes to last us a quite a while.

Week 8

Week 9

Week 10

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