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Team:KULeuven/NotebookDaily
From 2011.igem.org
Week 1
The first week in the wet lab started with the preparation of materials needed in future experiments. We made growth medium and agar plates containing antibiotics, which will be used to culture bacteria. Furthermore competent cells were prepared with the CaCl2 – method. We also resuspended the DNA of the biobricks necessary for our project as described in the iGEM guidelines. We managed to perform the first transformations with the competent cells and the resuspended DNA and thus concluded that the competent cells were functional.
Week 2
This week we wanted to purify the plasmid DNA from the bacteria grown in the first week. To do this we inoculated growth medium with antibiotics and a single colony. These cultures were incubated overnight at 37°C followed by a minipreparation to purify the DNA from the bacteria. Due to the low DNA yield we decided to repeat the minipreparation. This repetition succeeded in producing higher DNA concentrations.
Week 3
The goal this week was to bring different biobricks together in one plasmid (=ligation). We started with restriction of the biobricks that had been miniprepped the previous week. Some biobricks were successfully cut by the restriction enzymes whilst others weren’t. We performed gel purifications on the restricted fragments. However this didn’t result in high enough concentrations to start the ligation of biobricks.
Week 1
We installed the Matlab software and Simbiology toolkit, we watched some tutorial ‘webinars’ from Mathworks. In a meeting with the advisors, we learned more about the purpose and basics of modeling in synthetic biology. After downloading the projects of previous year teams of the KUleuven to understand the inner workings of their model.
Week 2
We made our own model in Simbiology of the freeze-antifreeze system. We still have to implement the kinetic parameters of the whole system to predict the outcome of the wet lab experiments. We wait for the promoters linked to GFP, synthesized in the wet lab, to check the kinetics of the promoters.
Week 3
Place text of third week modelling category.
