From Monday the 27th of June to Friday the 1st of July 2011

Monday

Tuesday

Culture of NM522 cells for later transformations and Curli for extraction.

Wednesday

Miniprep, digestion and electrophoresis of the Curli plasmid.

Thursday

CaCl2 chemical transformation of some iGEM kit distribution DNA :

Plate 1 :
18A (constitutive promoter, Amp )
2M ( strong RBS, Amp )
5L ( weak RBS, Amp )
22M ( RBS+YFP, Amp)

Plate 2 :
24E (YFP, Amp + Kan )
2L (GFP, Amp )

The local newspaper "VIVA" interviewed us. They ask about our project, our team and about iGEM's organization.

Friday

Miniprep of 6 iGEM parts. Digestion and electrophoresis.