From Monday the 18th of July to Friday the 22nd of July 2011

Monday

Start of liquid cultures containing iGEM parts or synthesized parts in order to extract DNA later

Tuesday

Midiprep to extract DNA from the previous liquid cultures.

Wednesday

Digestion of several biobricks : RBS, GFP, YFP, Curli, OmpR234. Electrophoresis to control the digestions.

Thursday

Standard or 3A ligation of the previously cut biobricks.TSS transformation into NM522.

Friday

Start of 5mL liquid cultures of selected individual colonies. Extraction of the plasmids to test the presence of the insert.