Team:Paris Liliane Bettencourt/Notebook/2011/07/22/

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Cyrille

Prearation of the pHM3 plasmid. This plasmid from H. Pfutzer contains 2 EcoRI excision site. One will be removed by quick change mutagenesis.

42 base oligo couple has been ordered from eurogentec. The protocol is the one http://labs.fhcrc.org/hahn/Methods/mol_bio_meth/quick_change.htmlhere

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