Gels for DspB

2011.07.03-2011.07.09


Gel proving the successful transformation of dspB containing plasmid into E. coli Top10. Lane 1: ladder; lanes 2-4: digests (EcoRI and PstI) of miniprep DNA from overnights of transformants. There is a clear band at the appropriate size for dspB in all of the experimental lanes.	Gel showing dspB with various promoter inserts as contrasted to a standard dspB with no promoter. Lane 1: dspB (WT) + P_rsaA; lane 2: with P_xyl; lanes 3,4: with BBa_K081005; lane 5: standard dspB; lane 6: ladder. Not all transformants show successful insertion of the desired promoter, but it is clear which were successful when compared to the standard dspB.	Gel of transformants that should contain both dspB and a promoter insert. Lane 1: ladder; lanes 2-4: dspB with P_rsaA; lanes 5-7: dspB with P_xyl; lane 8: standard dspB with no promoter.	Gel of more transformants of dspB with P_xyl. Lane 1: ladder; lanes 2-5: transformants; lane 6: standard dspB with no promoter.