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Team:Lyon-INSA-ENS/Realisation/Week5
From 2011.igem.org
Week 5
From Monday the 4th of July to Friday the 8th of July 2011
Monday
Additional minipreps of the same 6 parts. Digestion and ligation to obtain : RBS ( strong and weak ) + GFP, RBS + YFP. Culture of NM522 competent cells.
Tuesday
Transformation of the ligations into NM522 using a CaCl2 chemical transformation.
Wednesday
We shoot our "Cobalt Buster's Clip" which introduces, in a funny way, our team : students, instructors and advisors. We dedicate the all afternoon to this shooting.
