Team:Cambridge/Protocols/Transformation of B. subtilis

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Transformation of Bacillus subtilis

A technique used to introduce foreign DNA into competent Bacillus cells.

Theory

Practice

Add to a clean Eppendorf tube:

Reagent	Quantity
competent Bacillus cells	400μl
DNA	3μl

Incubate at 37ºC for 1 hour.
Plate on an agar plate containing antibiotic, which allows for selection of successful transformants.

Safety

All equipment (including gloves) that may have come into contact with the bacteria must be autoclaved for decontamination purposes.