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Team:Paris Bettencourt/ComS diffusion
From 2011.igem.org
The MeKS system is a noise tolerent bi-stable system that regulate the competence. However, this system is working in the stationnary phase, and we want to use it in the expodential phase.
To outsource the system, we have to work with a B. Subtilis strain CodY-, because CodY repress the MeKS system during the expodential phase, as well as thousands of stationnary phase gene. This strategy has been adopted because CodY regulation is so strong and touch so many gene, that it was faster to get rid of the regulator itself. The emittor cell has to be ComK- because we want to avoid the competence in a system that will modify the cell behaviour and may prevent the nanotubes from forming.
The design is the following:
