Team:Paris Bettencourt/ComS diffusion

From 2011.igem.org

The MeKS system is a noise tolerent bi-stable system that regulate the competence. However, this system is working in the stationnary phase, and we want to use it in the expodential phase.

For this step, we have to use a B. Subtilis strain CodY-, because CodY repress the MeKS system during the expodential phase, as well as thousands of stationnary phase gene. This strategy has been adopted because CodY regulation is so strong and touch so many gene, that it was faster to get rid of the regulator itself. The emittor cell has to be ComK- because we want to avoid the competence in a system that may modify the behaviour.