"
Team:MIT/nocontent
From 2011.igem.org
A hallmark of higher order organisms is the appearance of cell populations that interact with one another to form multi-cellular structures. However, although many scientists have studied these interactions, it has been very difficult to engineer multicellularity. This year, we aim to elucidate the engineering of multi-cellular mammalian systems through the study of two well-known and highly-conserved signalling motifs, the Notch-Delta signalling pathway and G-Protein Coupled Receptors. Our goal is to integrate the extracellular environment into our circuits to influence the emergence of specific self-assembling cell patterns by engineering these pathways. From these efforts, we will produce a proof of principle for tissue design and will look to pursue higher level tissue formation for application in therapeutics.
Safety
Would any of your project ideas raise safety issues in terms of:
researcher safety, public safety, or environmental safety? This summer, our team worked on cell patterning in mammalian cells. Part of our team worked with E. Coli in a BL1 lab, and a smaller group worked with mammalian cells in a BL2 lab. Both groups within the team followed to national and local safety protocols. Extra care was taken to not cross-contaminate lab spaces. Cross contamination from these settings were minimized by designating equipment specifically for mammalian cells and bacteria, as well as immediate change of personal protective equipment in moving between the different lab spaces.
Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues? If yes,
did you document these issues in the Registry? how did you manage to handle the safety issue? How could other teams learn from your experience? Neither our bacteria nor our mammalian cells contain BioBrick parts that code for hazardous proteins or molecules. We also determined that none of our circuits would survive if released outside the lab and pose no safety concerns to researchers, the public, or the environment.
Is there a local biosafety group, committee, or review board at your institution?
If yes, what does your local biosafety group think about your project? The EHS (Environment, Health, and Safety) Office is MIT's biosafety group that enforces lab safety in all labs on campus. They provide safety training, waste management services, and resources for safe lab practices. The safety training included general biosafety for researchers, managing hazardous waste, general chemical hygiene, lab-specific training, and bloodborne pathogen training. All undergraduates were trained by the EHS to work safely in BL1 labs, and students working with mammalian cells underwent BL2 lab safety training. EHS has been an integral part of our biosafety system and continues to be highly involved by conducting daily lab safety inspections.
Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?
Our MammoBlock construction standard not only aids in the construction of mammalian parts but also facilitates safer and easier storage of these parts. The MammoBlock standard uses bacterial entry vectors which allows mammalian parts to be stored in BL1 conditions.
This standard has allowed us to enter mammalian parts into the registry, and will facilitate the safe shipping and submission of mammalian parts constructed by future iGEM teams.
Our team is also greatly concerned with the safety issues regarding future iGEM research and competitions. This prompted us to sign up to teach a class for MIT’s Educational Studies Program summer HSSP session. Our class discusses the progress Synthetic Biology has made, controversies surrounding it, current research, and biosafety precautions and concerns. By teaching this course to middle school students we intend to get them excited about synthetic biology and prompt them to think about their own biosafety prior to getting more intense scientific training and experience in the lab. We hope that by exposing these children to Synthetic Biology and Biosafety Engineering at an early age, we can inspire these future iGEMers to proactively take a central role in enhancing biosafety and security in the iGEM competition.
