Team:Cambridge/Experiments/Initial Exercise Group control

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Positive Control Experiment

Construct Design

In the positive control experiment we replaced the Green Fluorescent Protein coding sequence with a coding sequence for mRUBY, which is a Bright Monomeric Red Fluorescent Protein. The picture below shows a map of the modified plasmid.

map of the modified plasmid with mRUBY insertion

The control experiment consists of 3 reactions

Reaction A: 1μl primer ruby F; 1μl primer ruby R; 1μl mRuby (Elliot)

Reaction B: 1μl primer Vector F; 1μl primer B reverse (provided); 1μl vector template

Reaction C: 1μl primer Vector R; 1μl primer A forward (provided); 1μl vector template