Team:Cambridge/Blog/Week 3

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Week 3 : 11th of July to 17th of July

Monday

The day begins with assembling our amplified DNA into working plasmids in order to transform Bacillus. Several mislabelled tubes and much all-round amateurishness later the task is complete and the day's brainstorming begins. Our possible projects are by now narrowed down to four which show some promise. It seems hopeful that a final choice can be made soon.

Tuesday

Our mini-projects continue apace with transformation of (hopefully) working plasmids into E. coli for replication. The team are introduced to the lab's high-tech portable dark room for working with fluorescence: a customisable, cubic, fully-opaque, stiffened wood-fibre local micro-environment device (known in the trade as a cardboard box).

The team's ideas converge further and seem to centre on [http://www.ncbi.nlm.nih.gov/pubmed?term=reflectin reflectin] - a protein with highly unusual properties isolated only relatively recently from [http://en.wikipedia.org/wiki/Bobtail_squid a class of squid]. Enthusiasm is higher than ever with a focus for our efforts.

Wednesday

Thursday

Friday

Saturday

Sunday


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