Team:Caltech/Week 2
From 2011.igem.org
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June 19Collection of soil and water samples from LA River June 20Lab walkthrough June 21MoBio Powermax Soil kit extraction of DNA from collected samples ResultsMoBio Extraction
Locations 3 and 8 were omitted from the MoBio Powermax Soil Extraction procedure because they were liquid samples. June 22Miniprep and sequencing of selected BioBricks ([http://partsregistry.org/Part:BBa_K123000 K123000], [http://partsregistry.org/Part:BBa_K123001 K123001], [http://partsregistry.org/Part:BBa_K123002 K123002], [http://partsregistry.org/Part:BBa_K123003 K123003]) Results
June 23Transform T7 polymerase ([http://partsregistry.org/Part:BBa_K145001 K145001]), mCherry ([http://partsregistry.org/Part:BBa_J06702 J06702]]), Lac Promoter ([http://partsregistry.org/Part:BBa_R0010 R0010]]), and double terminator ([http://partsregistry.org/Part:BBa_B0014 B0014] and [http://partsregistry.org/Part:BBa_B0015 B0015]) biobricks for test sequences for BisA and BisB degradation genes ([http://partsregistry.org/Part:BBa_K123000 K123000] and [http://partsregistry.org/Part:BBa_K123001 K123001]) ResultsWe determined that sequences for [http://partsregistry.org/Part:BBa_K123000 K123000] and [http://partsregistry.org/Part:BBa_K123001 K123001] have correct protein coding but different codons than in the sequences shown onlineJune 24Transform Tet repressible promoter [http://partsregistry.org/Part:BBa_R0040 R0040] ResultsWe determined that our competent cells are functional have a cfu/ug DNA of 6.6 x 10^6.June 25Take out plates from June 24
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