"

From 2011.igem.org

HOME

Contents 1 Temp Calender 2 Week 1 - June 1st-5th 3 Week 2 - June 6th-12th 4 Week 3 - June 13th-19th 5 Week 4 - June 20th-26th

Temp Calender

Week 1 - June 1st-5th

E. Coli
Comment Here

Cyano
Comment Here

Enzymology
Hexokinase coupled assay used to quantitate amounts of cyanobacteria glucose secretion. * Rxn: 1. glucose + ATP --Hx-> G-6-P + ADP * 2. G-6-P + NAD+ --G-6-PDeH-> G-6-P + NADH Reaction used Glucose assay kit (Genzyme) containing 2000U/L Hexokinase reagent and 4000U/L G-6-P DeH and measured glucose concentrations ranging from 0.05mM-0.25mM and absorbances were measured at 340.0 nm. Results: Final NADH concentrations were determined using the molar extinction coefficient and were proportionate to initial glucose concentrations. Discussion: Focus is now to measure not only glucose, but fructose concentrations as well as both with be secreted in the form of sucrose using a D-glucose/D-fructose assay (Megaenzyme.

Media

Week 2 - June 6th-12th

E. Coli
Comment Here
<p> Cyano
Comment Here

Enzymology
Comment Here

Media
Comment Here

Week 3 - June 13th-19th

Week 4 - June 20th-26th

==Week 5 - June 27th - July 3rd==