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From 2011.igem.org

Mentoring Notes

Contents 1 Week 1: 6/6-6/10 2 Week 2: 6/13-6/17 3 Week 3: 6/20-6/24

Week 1: 6/6-6/10

Finally iGEM kick off week arrived! I finished the BioBasics lectures with the entire team (both BU and Wellesley members were present). Following Orit's suggestion, I had the students break into teams and explain figures from a paper to the entire team to ensure students really did understand the concepts and results discussed in the paper.

Training for the wet team continued on Tuesday and Wednesday. During lab meeting on Wednesday, tasks were assigned to groups for building the first constructs the team will need to make (promoter-rbs-xFP-terminator).

These tasks included the following:

1. Choosing promoters, ribosomal binding sites and fluorescent proteins to build 4-part transcriptional units to generate fluorescence

2. Transforming the chosen BioBrick parts into Top10 competent cells

3. Miniprepping and carrying out restriction digests on these parts

4. Gel electrophoresis and extraction to confirm restriction digests

5. Ligations with the cut backbones and inserts

6. Repeat 1-5 until transcriptional units are made

Week 2: 6/13-6/17

I was at SB5.0 from 6/14-6/18 and was mentoring remotely.

Week 3: 6/20-6/24

I was away 6/22-6/25.