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From 2011.igem.org

Minipreps, digestion, purification and ligation

Axel

Minipreps

• on BBa_K090403 (vector), concentration of DNA=248.8ng/µL
• on BBa_K143079 (device with constitutive promoter and GFP), concentration of DNA=500.2ng/µL
• on BBa_B0015, concentration of DNA=408.1ng/µL
• on BBa_E1010 concentration of DNA=330.8ng/µL
• on BBa_K145001, concentration of DNA=572.8ng/µL

10 µL of minipreped DNA corresponds to approximately 125ng of DNA (see fluorescence of the 3Kb band of the ladder)

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digestion

• BBa_K090403 cut by EcoR1 and XbaI
  • BBa_K143079 cut by EcoR1 and SpeI
    • BBa_B0015 cut by EcoR1 and XbaI
      • BBa_E1010 cut by EcoR1 and SpeI
        • BBa_K145001 cut by EcoR1 and SpeI
        </p>

        gel electrophoresis at 0.6% of Agarose

        • BBa_K090403 cut by EcoR1 and XbaI
          • BBa_K143079 cut by EcoR1 and SpeI
            • BBa_B0015 cut by EcoR1 and XbaI
              • BBa_E1010 cut by EcoR1 and SpeI
                • BBa_K145001 cut by EcoR1 and SpeI