Team:UTP-Panama/Experiments2

GaTech 2010 Biobrick BBa_k41000

GaTech 2010 Biobrick BBa_K410000

Objectives:
1. Test the BioBrick operation to different substrates and temperatures:

a. Substrates:

• LB
  
• Minimal essential medium
  
• Saline solution
  
• Lunar land
  

b. Temperatures:

• 8°C
  
• 23°C
  
• 37°C
  

2. Improve the characterization of the BioBrick tested in other substrates to study the performance of the promoter.
3. Define the Biobrick promoter strength at the temperatures and selected substrates.

Methodology

We chose 4 temperatures:
• 5-10°C
• 10-20°C
• 20-30°C
• 37°C

These temperatures were selected so we could evaluate the response to cold shock in various ranges of our BioBrick. Since the lab equipments we had available couldn’t reach temperatures between 10-20°C this temperature was not used during the experience. Finally, the lab equipment allowed us to make the experiments in: 37°C, 23°C and 8°C.

We also chose 3 different Growth Media:
• Saline Solution
• Liquid LB
• Minimal Media

These growth medias where selected to evaluate the response of our BioBricks in different metabolic pathways. We prepared 9 15mL Falcon Tubes, these tubes contained:
• First 3 (one for each temperature): 10mL of Saline Solution + a colony of the BioBrick.
• Second 3 (one for each temperature): 10mL of Minimal Media + a colony of the BioBrick.