Team:Queens Canada/Parts/Registry

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Modular GPCR Constructs

In approaching our chemotaxis project, several G-protein coupled receptors were cultivated from multiple mammalian sources.

BBa_K631005 - NPY1R_Mus:The coding sequence for the NPY1R protein. This is a GPCR from Mus musculus.
BBa_K631006 - NPY1R_Rat:The coding sequence for the NPY1R protein from Rattus norvegius. This is also a GPCR .
BBa_K631007 - ADBR2: The coding sequence for ADBR2, a mouse GPCR that binds phenol (albuteroll). Length: 1257
BBa_K631009 - OX1R: Coding sequence for OX1R, a mouse GPCR that binds cholorobenzoic acid.
BBa_K631010 - MARM: Coding sequence for MARM1, a rat receptor that binds phenol (hydroquinone).
Regulatory Constructs for C. elegans

BBa_K631013 - RFP: Red fluorescent protein for the reporter system in our worm.
BBa_K631036 - eCFP:UNC-54: Eukaryotic cyano-fluorescent protein for the reporter system in our worm. Length: 1629.
BBa_K631039 - nahD: The coding sequence for the nahD protein, from P. peutida, capable of degrading napthalene.
BBa_K631035 - Str2: A promoter for AWC neurons, normally used for a 7-transmembrane olfactory receptor. We can use this as a promoter to integrate non-native GPCRs into the AWC neuron membrane.
BBa_K631001 - ODR-7: A promoter for a 7-transmembrane olfactory receptor in AWA olfactory neurons. This can be used for integrating non-native GPCRs. Length: 1503
BBa_K631002 - let-858: A promoter for a protein essential for the development of the worm. We used this in one of our composite parts to express fluorescence in the worm.
BBa_K631003 - GPC-1: A promoter for one of the gamma sub-units of the GPCR binding proteins. We used this for our fluorescence as well. Length: 2000
BBa_K631004 - GCY7: A promoter for a membrane guanylyl cyclase protein. This can be used for a non-native receptor or fluorescence reporter.

Composite Constructs

BBa_K631031 - ODR7:OX1R:UNC-54: A composite part designed to place the non-native OX1R into the neurons of the worm using the ODR-7 promoter for the ability to detect cholorobenzoic acid.
BBa_K631032 - ODR7:ADBR2:UNC-54: A composite part designed to place the non-native ADBR2 into the neurons of the worm using the ODR-7 promoter for the ability to detect phenol (albuteroll). Length: 3666
BBa_K631033 - GCY7:ADBR2:UNC-54: Another composite part with ADBR2, under the GCY7 promoter.
BBa_K631037 - GPC-1:eCFP:UNC-54: This is a composite part for our reporter system using cyano-fluorescent protein under the GPC-1 promoter. Length: 3787
BBa_K631034 - let858:RFP:UNC-54:This is a composite part for our reporter system using red fluorescent protein under the let858 promoter.
BBa_K631038 - GCY-7:eCFP:UNC-54: This is a composite part for our reporter system using cyano-fluorescent protein under the GCY-7 promoter.
BBa_K631039 - nahD: The coding sequence for the nahD protein, from pseudomonas peutida, capable of degrading napthalene. Expressing this in our worm may give it the ability to degrade naphthalene.
Complete List of parts

<groupparts>iGEM011 Queens_Canada</groupparts>

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