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RecA: Week 7, June 26-July 1
From 2011.igem.org
Contents |
Sunday, June 26
Insert RecAI into K3 Vector, Day 1
The RecAI PCR product was purified, then digested with SpeI and XbaI. The K3 vector plasmid (extracted on 6/24) was digested with SpeI and XbaI as well. The digests were ligated together, the ligation was transformed into Escherichia coli cells, and the cells were plated onto kanamycin resistant plates.
