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Team:Lyon-INSA-ENS/Realisation/Week19
From 2011.igem.org
Week 19
From Monday the 24th of October to Friday the 28th of October 2011
Monday
Tuesday
Wednesday
Thursday
Fluorescence Test - Cobalt induction selectivity
Studie of the induction of the pLac promoter by cobalt with a kinetic studie.
2 people are repeating the same 4 erlen of culture with the M63G medium:
- MC4100/p85 + amp + 25µM Co
- MC4100/p85 + amp
1 ml of ampicilline is added
The culture is sed by 1mL of a overnight preculture.
As the value of the fluorescent output should be 0, an induction by IPTG will be done in order to check that the pLAc promoter is functionnal and that it is just non responsive to cobalt.
Friday
