Team:uOttawa

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Saccharomyces cerevisiae (budding or brewer's yeast) has emerged as an important micro-organism in biomedical research and industry. Not only is this yeast an important model organism due to its homology to higher eukaryotes, but it sees wide commercial application as well. Small molecule biofabrication, biofuel production, food and beverage production all make heavy use of the humble yeast. For this reason we feel that it is essential that synthetic biology grapple with the manipulation, characterization, and optimization of gene expression and regulation in S. cerevisiae. To this end, team uOttawa set out to build a novel yeast chassis to allow the robust characterization of BioBricks in yeast. We have also submitted several BioBricks optimized for use in S. cerevisiae. Finally, we succeeded in developing a novel assembly protocol that will greatly improve upon existing BioBrick assembly protocols, not just for those manipulating yeast but for the whole BioBrick community. So raise a glass to your favourite single-celled eukaryote, and let yeast leaven your spirits.