Team:Tec-Monterrey/projectmodeling/construct3

Our third construct ompA+sacC has the same promoter as the last two (P_BAD promoter, BBa_K206000, and its repressor protein araC, BBa_I13458 ) We decided to use an already reported part, membrane protein ompA and a signal peptide of lpp (BBa_K103006). We decided to try out our extracellular invertase “sacC” (BBa_K633003) cloned out of Zymmomonas Mobilis in our university. The importance behind this part is that it’s a monomeric enzyme, able to produce both glucose and fructose from sucrose, an important approach for high fructose syrups.

Kyono,K., Yanase,H., Tonomura,K., Kawasaki,H. and Sakai,T.”Cloning and characterization of Zymomonas mobilis genes encoding extracellular levansucrase and invertase” Biosci. Biotechnol. Biochem. 59 (2), 289-293 (1995)

Kannan,R., Mukundan,G., Ait-Abdelkader,N., Augier-Magro,V., Baratti,J. and Gunasekaran,P.”Molecular cloning and characterization of the extracellular sucrose gene (sacC) of Zymomonas mobilis” Arch. Microbiol. 163 (3), 195-204 (1995)