Team:Peking R/Notebook/YXW

I am in charge of the construction of c-di-GMP and theophylline ribozymes, focusing on the response properties of these ribozymes to corresponding ligands ---- c-di-GMP & theophylline. I am also the designer of our team's shirt.

7.1--7.7 •Positive transformation of the parts which may be used in our project from 2011 Distribution.

7.8-7.16 •Primary construction of the part about c-di-GMP responsive ribozyme. At first, assembly the c-di-GMP responsive ribozyme with GFP using OE-PCR (pBAD+c-di-GMP ribozyme+GFP+ter B0015). Because of the delayed primers, the problems of DNA purification kit and gel extraction kit, the ignorance of the question on fidelity of Taq enzyme, these experiments have been done many times, so they’re finished a little late. Whatever, WT、M6、M8 and GFP have been OK now. •The growth curve determination of DH5α in media of LB.

7.17-7.24 •Assembly the c-di-GMP responsive ribozyme (WT/M6/M8) with GFP using OE-PCR successfully. •Some comparisons between different kits.

7.25-7.31 •Violacein Separation of individual genes in the violacein metabolic pathway ——vioA/B/D/E. The primers of vioC seem to have a prefer annealing ability with a particular sequence within vioC. Testing and optimizing the protocols of PCR.

8.1-8.5

8.6-8.13

8.14-8.18

8.19-8.25

8.26-8.31

9.1-9.8

9.9-9.15

9.16-9.22

9.23-9.30

10.1-10.4