We have successfully constructed a novel system in which bacterial colonies will automatically divide into two parts after certain time. We have successfully assembled riboswitches finely tuned by small molecules and toggle switches pushed on and off while memorizing current state.
Here we present modeling of our system in order to verify our design and help constructing new systems. Besides we have detailed analysis of [https://2011.igem.org/Team:USTC-China/Drylab/riboswitch riboswitches]invovled in our system.