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Team:NYMU-Taipei/results/immunological-solution1
From 2011.igem.org
tetR+RBS+GFP
These are measurements of construct tetR+RBS+GFP under fluorescence microscope. The left graph is control group of E.coli strain DH5a. The middle is tetR+RBS+GFP in plasmid c3 in E.coli , while the right is plate without any bacilli. All graphs are measured in fixed exposure time. Construct of tetR+RBS+GFP which emits light is thought to be a leak phenomenon of plasmid, for RE digest has proved the sequence length right, and further sequencing work is under operation.
The expression of GFP is measured precisely under excitation wavelength from 475nm to 501nm, given the absorption wavelength 511nm. In our measurement, wavelength over 493nm yields high background, resulting in values of control group surging far beyond normal condition. Thus, 493nm is suggested as the best excitation wavelength, as it presents the highest emission light and the lowest of control group.
