Team:HKU-Hong Kong/Parts

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Sample Data Page
Sample Data Parts.png

By expressing the tetR:H-NS fusion proteins, the tetR part of the proteins wound recognize and specifically bind to the tetR binding region (tetO2). There are two tet-R binding sites as tet-R form a dimer as in the tet repressor- operator system. As the fusion proteins bound to tet-O, it is expected that the H-NS part of the fusion protein would attract and oligomerize with other native H-NS proteins inside the cell. With the oligomerization of the fusion proteins and native H-NS, the DNA covered by the oligomers is expected to trap the RNA polymerase during transcription, thus gene (GFP) repression is achieved.


Parts that we have created:

(The favorite parts are selected based on the potential to be used in our Super silencer repression system)

BBa_K544001 [[[http://partsregistry.org/BBa_K544002|BBa_K544002 ]] [[[http://partsregistry.org/BBa_K544003|BBa_K544003 ]]
[[[http://partsregistry.org/BBa_K544012|BBa_K544012 ]] [[[http://partsregistry.org|BBa_K544011 ]] [[[http://partsregistry.org/|BBa_K544013 ]]
[[[http://partsregistry.org/BBa_K544014|BBa_K544014 ]] [[[http://partsregistry.org/BBa_K544015|BBa_K544021 ]]