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From 2011.igem.org

Introduction

        According to the five clear quorum sensing system we summarize in the New QS Device, we could build a promoting cycle imitating the Wu xing’s promoting cycle.

General mechanism

        In the promoting cycle, Every device of our five element cycle is composed by 4 basic segments: activator protein production; AHL synthetase production; protein LeuB production and reporter fluorescent protein expression.

        The first promoter is ptetR，as the E.coli chassis we used do not contains the tetR, so it have a constitutive expression, generating the activator protein, like luxR，lasR, cinR, sinR, rhiR. When the input signal, namely AHLs, meet the activator. They will bind with each other and dimerize, forming a dimmer, which will bind the cassette in the second promoter in the picture, activating the downstream genes’ expression.