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From 2011.igem.org

The experiment of screening defective bacteria

Step-I—Screen by streak cultivation

        We get 10 single colonies of HB101 by streaking. And they’re labeled for No.1—No.10 and separately streak cultured. So on the square plates, we get ten parts which are separately culturing ten bacteria colonies. Then we prepare essential culture medium and complementary culture medium to culture them and use BL21 as the contrast to screen the defective bacteria. HB101 can’t synthetize Proline and thiamine. So we have to add these two elements.
Results in theory: