Team:NYMU-Taipei/chassis

Cultivating Magnetospirillum magneticum AMB-1 Actually, it wasn't difficult...

Like E-coli, AMB-1 also grows in liquid medium, and this medium called Magnetic Spirillum Growth Medium (MSGM). In the medium, we added nutrient, iron source (supplying AMB-1 in magnetite formation within magnetosomes), vitamin, and mineral solution.

MSGM's ingredients

(In grams per liter)

●succinic acid             0.74 g

●KH2PO4                    0.68 g

●sodium nitrate            0.12 g

●sodium thioglycolate      0.1 g

●Wolfe’s vitamin solution  10 ml(stock)

●Wolfe’s mineral solution  5  ml(stock)

●ferric quinate solution   2  ml(0.27 g of FeCl3 and 0.19 g of quinic acid in 100 ml water)

We also refered a paper which added Polypeptone , Yeast extract and L-cysteine into MSGM. Which called En-rich MSGM.

● ●