Team:XMU-China/Notebook

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Notebook

Week 1(3rd Apr.—9th Apr.)

Aim:

By replacing the promoter of the existing BioBrick BBa_F2621 with Placo-1, the expression of the downstream sequence can be controlled by adding IPTG.

Performance:

Constructing the new BioBrick BBa_K658000 (The figure below and sequence analysis can indicate it is correctly constructed.) Testing the expression of BBa_K658000 by adding IPTG (We thought the part didn’t exist in the registry and we had constructed a new part. But, afterwards, we found it(BBa_F2622) did exist! )

Fig.1

Week 2W(10th Apr.—16th Apr.)

Aim:

By combining the BioBrick BBa_R0011, BBa_F1610 and BBa_K65800, we can get a new BioBrick which is part of the bacteria population-control device we have planned to design. Performance:

Constructing the new BioBrick IR(BBa_K658012)