Team:ZJU-China/Notebook/September

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Labnote

Biobrick group | Biofilm group

This group...........

May&June

In the two months we discussed some new ideas and finally decided our project
>> Click to see our brainstorm

July

In this month we......
>>Click to see

Week9

Day

Note

Aug.29th Monday

• Meeting. Summarize all the experiment work so far.

• Cut: 22M(X+P), 1C3(E+P)

Aug.30th Tuesday

Aug.31st Wednesday

• Cut vgb+YFP+tetR, fdhF+RFP+tetR.
• Run the digestion results

• Culture the positive results

Sept.1st Thursday

• Miniprep: Y#4, R#2/3/4/5
• Cut Y#4, R#2/3/4/5

•Run the gel.
• Send R#3,Y#4 sample to sequencing

• Hypoxia culture: RFP3, RFP4
• Preserve Y#4, R#2/3/4/5

Sept.2nd Friday

• Phusion PCR

• Hypoxia Culture: R, Y
• Run the PCR results. Bands are confirmed right.

• Purification: vgb, YFP, tetR.

Sept.3rd Saturday

• Culture: YFP, RFP, A25
• Culture in micro-oxygen: YFP, RFP, A25

• Culture in slope medium: RFP, YFP, A25
• Hypoxia culture: RFP, YFP, A25

• Check the fluorescence.
• Neither RFP in hypoxia or oxygen condition. Little YFP both in hypoxia and oxygen.

Sept.4th Sunday

• Miniprep: RFP3, YFP4

• Cut: RFP3, YFP4, !C3.

Week10

Day

Note

Sept.5th Monday

• Run the gel: Yu’s PCR results, 1C3, RFP3, YFP4
• Purification: RFP3, YFP4

• Ligation: RFP3+1C3, YFP4+1C3
• Hypoxia culture: RFP3, YFP4, A25.

• Transform the ligation results.
• Confirmed RFP expression only in hypoxia condition.

Sept.6th Tuesday

• Check the RFP, YFP plates.

• Colony PCR

Sept.7th Wednesday

• Culture YFP1/2, RFP3/4

Sept.8th Thurday

• Preserve the strain of RFP1, YFP1/2 in 1C3.
• Miniprep the remains.

• Cut: YFP1/2, RFP1

• Run the digestion results.
• Culture RFP2

Sept.9th Friday

• Miniprep: RFP2, YFP2, RFP1

• Cut them and run the gel.

Sept.10th Saturday

• Cut YFP(1A3), RFP(1A3) with S+E

• Purification: RFP(1A3), YFP(1A3)

• Ligation: RFP+1C3, YFP+1C3

• Transform the ligation results.

Sept.11th Sunday

• Check the plates.

• Culture lig RFY+YFP+IC3
• Culture 1K3

• Colony PCR: RFP(1C3)， YFP(1C3)