Team:Peking S/lab/notebook/fjc

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Jingchen Feng's Notebook

summary

I am mainly responsible for the modeling work of our project. I model our balancer and give simulation result of balancer and XOR gate. Besides, under the guidance of Xiao Qing and Yiwei Chen I help constructing sRNA-dependent competitor. I also participate in the microfluidic work.

Contents

6.24-7.5

Molecular cloning training . such as transformation and DNA ligation and Enzyme digestion.


7.6-7.7


Obtaining Streptomyces strain from professor Honglong and acquired some skills in Streptomyces culturing.


7.8-7.12


Usinig PCR strategy to get a-factor generator gene afsA and a-factor receptor gene arpa from Streptomyces genome.


7.13-7.23


Using different polymerases(prime star、esay pfu、TAG ) in PCR to gain afsa and arpa genes from the Streptomyces strain. And we finally got those two genes by changing many PCR parameters.


7.24-8.13


Constructing the A-factor receive system including constitutively expressed arpA and a gfp whose expression is controlled by arpA and afsA synthesized molecules.


8.14-9.5


Testing A-factor system performance.


9.6-9.18


Construction cinI/cinR system mainly contains constitutively expressed cinR and a gfp controlled by cinR and cinI synthesized chemical molecules.


9.19-10.1


Characterization of cinI/cinR system.