Team:Panama/16 June 2011

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 We autoclaving 1.5ml eppendorff tubes and then were treat at 800C for 2h in the microwave for nuclease elimination.  Orlando prepare chloranfenicol (100µg/ml)  We pass our Biobrick from Agar medium +chloranfenicol to LB medium + Chloranfenicol.  Sandra use as negative control : 200µl plasmid (RBS+T) in medium with Amp and inoculate in LB medium with Chloranfenicol. No colonies have to grow because not have resistance for chloranfenicol  Incubate at 370 C in shaker.

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