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Team:Calgary/Notebook/Protocols/Process20
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Notebook
Post-Regionals
Journal
- Defining Objectives
- Techniques Workshop
- Bioinformatics & qRT-PCR
- Sensory Element Fishing
- Electrochem - CPR Oxidation
- Electrochem - Reporter Gene
- Pseudomonas Tools
- Microalgae Tools
- NA Viability Assays
- May - July Group Journal
Protocols
Safety
Preparing Glycerol Stocks
Reagents and Materials
- Overnight bacterial growth
- screw cap tubes
- glycerol
Protocol
- Pipet 0.5mL of 50% glycerol into 3 1.5mL screw cap tubes
- Add 0.5mL of overnight culture to each tube.
- Pipet up and down to gently mix.
- Flash freeze in liquid N2 or dry ice/ethanol bath.
- Place in -80°C freezer when frozen.
