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• iGEM

• Overview
• Promoter Project
  • Sensory Element Fishing
  • Bioinformatics and RT-PCR
• Reporter Project
  • Preliminary Testing
  • Characterization
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• Microalgae Tools
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Reporter: For the electrochemical reporter we would like to further characterize our system, especially with regards to the optimal buffer solution that a sample should be placed in. After this has been done we would like to test a variety of substrates with lacZ as well as other genes to determine what the optimal reporting molecule is. Also, seeing if we can develop a smaller and cheaper version of the setup is an option we would like to explore.

qRT-PCR: On the RT-PCR side of the promoter search there are a few more points to test. Although we have identified a potential candidate gene, up-regulated in the presence of NA's, the next step is to test if the response observed can be duplicated with a general stress, such as hydrogen peroxide. Another thing to test is the specificity of the observed response, to see if various kinds of naphthenic acids can elicit the same response.

"Promoter Fishing": For this side of the project we need to optimize the interaction between the streptavidin beads and our biotinylated compound. To do this we are going to have to find a way to efficiently seperate our compound from the solution that it is currently in without damaging the biotinylated compound. After this is done we will be able to perform the IPs that we have envisaged.

Chassis: In terms of tools, further chaacterization of the HspA70/RbcS2 promoter and algal luciferase is needed. The addiiton of a few more selectable markers suitable for use in algae. Ultimately, we also want to finish the construction and screening of chloroplast and nuclear promoter libararies in Dunaliella tertiolecta.