Team:Panama/3 September 2011
From 2011.igem.org
We repeated the PCR of the Biobrick using other master mix, with the miniprep´s products obtained from the Biobrick ordered at MIT.
We prepared a chloramphenicol stock (50mg/mL), which was stored at -20 °C.
Primers used for PCR:
• RhT2b+RhT2a
• RhTbio2a+RhTbio2b
• RhTbioF1b+RhTbioF1a
• RhT1a+RhT1b
Program used for PCR: