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Team:Colombia/Notebook/Plasmids
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Contents |
Plasmids
The Plasmids will be constructed as shown below
Plasmid 1
Plasmid 1 mission is to recognize the presence of chitin and to up regulate the promoter of the chitinolitic operon when chitin is abundant in the media. We´ve extracted and amplified chitoporin, chitin sensor and chitin binding protein (CBP) from Vibrio fischerii and aimed to create the bricks needed for the assembly of the plasmid that can recognize and break the chitin association.
Plasmid 2
Plasmid 3
In a nutshell, P3’s mission is that of interpreting P2’s autoinduction signals (LuxI) in a way so that it responds with an appropriate production of the plant’s Systemic Aquired Resistance (SAR) stimulating factor (Salicylic Acid, SA). We have also included a negative feedback loop in order to control possible gene overexpressions by introducing a LuxI activated lactonase.
Test Plasmid
Building a whole working system is the challenge our group faces everyday. But, as important as the assemblage itself is to test our assumptions about how the designed parts work individually. Currently, this little group comprised by David Ayala-Usma and Óscar Ortega Sandoval with collaboration of Paola Reyes and Nathaly Montenegro, is working on the induction of the Systemic Acquired Resistance of Nicotiana benthamiana (model organism) by Salicylic Acid BioBrick (BBa_J45320) and the response of the designed chitin-induced promoter to (GlcNAc) polymers.
