Team:Queens Canada/Parts/Contributions

Materials - Thermocycler
- PCR Tubes (0.2mL)
- KAPA HiFi™ Hotstart Kit
- 10 µl of each of the 2 linear BioBrick parts to be ligated
� - 10 µM solution of left primer of BioBrick part A
- 10 µM solution of right primer of BioBrick part B
- Fermentas FastDigest SpeI system
- Fermnetas FastDigest XbaI system
- KAPA Fast Ligase system
- TBE (pH = 8.0)
- 3 µl of Ethidium Bromide
- Biotechnology grade Agarose
- Gel Box
- Power Supply
- Gel Supply
- Loading Dye
- Bio Basics EZ-10 PCR Product Purification Kit
- Transilluminator

Method