Reporter: Week 11 July 26-30

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Contents

Tuesday, July 26

Clone tev protease (K316017) into K3

Wednesday, July 27

All colonies from July 26 clone=red, retry clone tev protease into K3

Clone XylE and GFP into K3

Prepare cultures of K316009 for catechol assay

Thursday, July 28

Colony PCR GFP+K3, XylE+K3, RecA+K3 and tev protease+K3 Results: GFP+K3 and tev protease+K3 failed, all else worked

Test K316009 with catechol assay Results: no difference between induced and uninduced cultures

Friday, July 29

clone tev protease into K3

J23100+RBS34 GFP+tev and cI cleavage sites

Saturday, July 30

Colony PCR of J23100+RBS34, GFP+tev cleavage site, GFP+cI cleavage site Results: all failed

XylE+3 linkers LacZα+linkers

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