Team:Calgary/Notebook/Protocols/Process7

From 2011.igem.org

Revision as of 01:38, 28 September 2011 by Yqi2011 (Talk | contribs)


Plasmid Extraction

This protocol will purify plasmids from bacteria, though identification of plasmids will need to be confirmed by PCR.


Reagents

  1. HEPES (N-[2-hydroxyethyl] piperazine N'[2-ethane-sulfonic acid])
  2. Sorbitol
  3. Percoll
  4. PEG 6000
  5. Ficoll
  6. Bovine serum albumin (BSA)
  7. Isoascorbic acid
  8. Sodium pyrophosphate
  9. Glutathione
  10. MgCl2.6H2O
  11. MnCl2
  12. Sodium-EDTA
Equipment

Retrieved from "http://2011.igem.org/Team:Calgary/Notebook/Protocols/Process7"