Team:UT Dallas/week9
From 2011.igem.org
Week 9
9/6/11 | Incubation: Germany Parts=[pHKToxR'MalE, pHKToxR'MalE/ZIP, pHKToxR'REI-T39K, pHKToxR'MalE/ZIP L267S, pHKToxR'HisJGCN] (FHK12) CheY, PyeaR-GFP-RBS-TetR-term, scp-GFP-RBS-TetR-Term |
9/7/11 | Glycerol Stock/Miniprep: [pHKToxR'MalE, pHKToxR'MalE/ZIP, pHKToxR'REI-T39K, pHKToxR'MalE/ZIP L267S, pHKToxR'HisJGCN] Incubation: pYeaR-GFP-RBS-TetR-Term, scp-GFP-RBS-Tetr-Term, AHL inducible Colicin, Pcst-RBS-LuxI-Term |
9/8/11 | Dilution of Pcst-RBS-:uxI-Term, AHL induible colicin Incubation: DATA COLLECTION=GFP quantification [(a) Psct-RBS-LuxI-Term vs. (b) AHL inducible colicin](DH5-alpha) |
9/9/11 | Full Digestion: FGFR, RBS-GFP-TetR-Term, CheY, CheZ*, ctx-CheZ Test Digestion: Pyear-GFp-RBS-TetR-Term, scp-GFP-RBS-TetR-Term |
9/10/11 | Colony PCR: ToxR-FGFR, ctx-CheZ*, ctx-CheZ |