From 2011.igem.org
{{Team:Calgary/Notebookbar|
TITLE=cDNA Synthesis/Reverse Transcription Reaction|
BODY=
cDNA Synthesis by Reverse Transcription |
Component |
Volume |
RNA (40ng – 10ng to 1μg total RNA/rxn) |
variable |
Nuclease free water |
variable |
qScript cDNA synthesis mix |
4 uL |
Final Volume |
20 uL |