Team:Colombia/Notebook/Protocols

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PCR for 16s (10 µl)

Reactives µl µl Buffer 2.5 1 MgCl2 2 0.8 DNTP´s 0.5 0.2 P1492R 0.5 0.2 P27F 0.5 0.2 Taq 0.25 0.1 DNA 2 0.8 H2O 17.75 6.8 Total Mix 26 10