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Team:MIT/Results/
From 2011.igem.org
Parts Constructed
Type: Regulatory Length: 1275
This part encodes a promoter with low-level, constitutive activity that can be repressed by variants of the LacI transcriptional repressor. Repression by LacI-KRAB through chromatin packing is quite effective.
Parts Characterizations
The characterization of newly constructed biological parts is ADD TO BLURB
List of characterizations
- rtTA3/TRE promoter
- Gal4/UAS promoter
- LacI/Hef1a-LacO repressor
- Delta-Notch interaction
- CMV-TetO promoter
- Mnt-VP16/Mnt promoter
- CI434-VP16/CI434 promoter
- Gal4→LacI¬rtTA3→Reporter cascade (!)
rtTA3/TRE promoter
Explanation:
EXPLAIN HERE
Gal4/UAS promoter
Explanation:
EXPLAIN HERE
LacI/Hef1a-LacO repressor
Explanation:
EXPLAIN HERE
Delta-Notch interaction
Explanation:
EXPLAIN HERE
CMV-TetO promoter
Explanation:
EXPLAIN HERE
Mnt-VP16/Mnt promoter
Explanation:
EXPLAIN HERE
CI434-VP16/CI434 promoter
Explanation:
EXPLAIN HERE
Gal4→LacI¬rtTA3→Reporter cascade
Explanation:
EXPLAIN HERE
Cell Patterning
Patterning!Attributions
Our instructors were very helpful not only in giving feedback on our designs, cloning strategies, and data, but also in training us for lab work. The training for tissue culture work was conducted by Linda Stockdale in the Griffiths lab. Gibson assembly techniques and FACS training from Deepak Mishra, one of our instructors.
Other than the initial training, all work was done by our undergraduate team.
Special credit belongs to Semon Rezchikov and Jenny Cheng for simulations and modeling, and Tiffany Huang for wiki design.