Team:Northwestern/Results/Data

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fig1


Favorite Parts


  • [http://partsregistry.org/Part:BBa_K575024 BBa_K575024 Main Page] This construct consists of the gene for GFP, regulated by a promoter that is induced by the dimerization of lasR and 3-oxo-C12-HSL (a signaling molecule produced by Pseudomonas Aeruginosa), as well as a constitutively expressed gene for the lasR receptor protein. Cells transformed with this construct would ideally fluoresce green in the presence of 3-oxo-C12-HSL, thereby indicating the presence of Pseudomonas Aeruginosa. We have shown that our cells do in fact fluoresce green when various concentrations of autoinducer is present. This means that the lasR receptor protein is successfully being produced and the promoter induced by the lasR/3-oxo-C12-HSL complex is working properly.


  • [http://partsregistry.org/Part:BBa_K575032 BBa_K575032 Main Page] This construct consists of the gene for GFP, regulated by a promoter that is induced by the dimerization of rhlR and C4-HSL (a signaling molecule produced by Pseudomonas Aeruginosa), as well as a constitutively expressed gene for the rhlR receptor protein. Cells transformed with this construct would ideally fluoresce green in the presence of C4-HSL, thereby indicating the presence of Pseudomonas Aeruginosa. We have shown that our cells do in fact fluoresce green when various concentrations of autoinducer is present. This means that the rhlR receptor protein is successfully being produced and the promoter induced by the rhlR/C4-HSL complex is working properly.


  • [http://partsregistry.org/Part:BBa_K575046 BBa_K575046 Main Page] This construct consists of the gene for GFP, regulated by a promoter (extracted from the Pseudomonas genome) that is induced by the dimerization of rhlR and C4-HSL (a signaling molecule produced by Pseudomonas Aeruginosa), as well as a constitutively expressed gene for the rhlR receptor protein. Cells transformed with this construct would ideally fluoresce green in the presence of C4-HSL, thereby indicating the presence of Pseudomonas Aeruginosa. We have shown that our cells do in fact fluoresce green when various concentrations of autoinducer is present. This means that the rhlR receptor protein is successfully being produced and the promoter induced by the rhlR/C4-HSL complex is working properly.


Well-characterized Parts


  • [http://partsregistry.org/Part:BBa_K575008 BBa_K575008 Main Page] This construct successfully functioned as part of Part [http://partsregistry.org/Part:BBa_K575024 BBa_K575024].
  • [http://partsregistry.org/Part:BBa_K575009 BBa_K575009 Main Page] This construct successfully functioned as part of Part BBa_K575028.
  • [http://partsregistry.org/Part:BBa_K575012 BBa_K575012 Main Page] This construct successfully functioned as part of Part BBa_K575032.
  • [http://partsregistry.org/Part:BBa_K575013 BBa_K575013 Main Page] This construct successfully functioned as part of Part BBa_K575036.
  • [http://partsregistry.org/Part:BBa_K575023 BBa_K575023 Main Page] This is a promoter region extracted from the Pseudomonas Aeruginosa genome. It is activated by the dimerization of rhlR and C4-HSL.
  • [http://partsregistry.org/Part:BBa_K575028 BBa_K575028 Main Page] This construct enabled cells to physically convey the presence of Pseudomonas Aeruginosa by fluorescing green in the presence of 3-oxo-C12-HSL.
  • [http://partsregistry.org/Part:BBa_K575033 BBa_K575033 Main Page] This construct enabled cells to physically convey the presence of Pseudomonas Aeruginosa by fluorescing green in the presence of C4-HSL.
  • [http://partsregistry.org/Part:BBa_K575036 BBa_K575036 Main Page] This construct enabled cells to physically convey the presence of Pseudomonas Aeruginosa by fluorescing green in the presence of C4-HSL.
  • [http://partsregistry.org/Part:BBa_K575037 BBa_K575037 Main Page] This construct enabled cells to physically convey the presence of Pseudomonas Aeruginosa by fluorescing green in the presence of C4-HSL.
  • [http://partsregistry.org/Part:BBa_K575042 BBa_K575042 Main Page] This construct successfully functioned as part of Part BBa_K575046.
  • [http://partsregistry.org/Part:BBa_K575043 BBa_K575043 Main Page] This construct successfully functioned as part of Part BBa_K575047.
  • [http://partsregistry.org/Part:BBa_K575047 BBa_K575047 Main Page] This construct enabled cells to physically convey the presence of Pseudomonas Aeruginosa by fluorescing green in the presence of C4-HSL.


Characterization of pre-existing Parts


  • [http://partsregistry.org/Part:BBa_C0071:Experience BBa_C0171 Experience]
  • [http://partsregistry.org/Part:BBa_C0079:Experience BBa_C0179 Experience]
  • [http://partsregistry.org/Part:BBa_R0071:Experience BBa_R0071 Experience]
  • [http://partsregistry.org/Part:BBa_R0079:Experience BBa_R0079 Experience]


Other Parts
  • [http://partsregistry.org/Part:BBa_K575004 BBa_K575004 Main Page]
  • [http://partsregistry.org/Part:BBa_K575005 BBa_K575005 Main Page]
  • [http://partsregistry.org/Part:BBa_K575006 BBa_K575006 Main Page]
  • [http://partsregistry.org/Part:BBa_K575007 BBa_K575007 Main Page]
  • [http://partsregistry.org/Part:BBa_K575010 BBa_K575010 Main Page]
  • [http://partsregistry.org/Part:BBa_K575011 BBa_K575011 Main Page]
  • [http://partsregistry.org/Part:BBa_K575014 BBa_K575014 Main Page]
  • [http://partsregistry.org/Part:BBa_K575015 BBa_K575015 Main Page]
  • [http://partsregistry.org/Part:BBa_K575020 BBa_K575020 Main Page]
  • [http://partsregistry.org/Part:BBa_K575021 BBa_K575021 Main Page]
  • [http://partsregistry.org/Part:BBa_K575022 BBa_K575022 Main Page]
  • [http://partsregistry.org/Part:BBa_K575025 BBa_K575025 Main Page]
  • [http://partsregistry.org/Part:BBa_K575026 BBa_K575026 Main Page]
  • [http://partsregistry.org/Part:BBa_K575027 BBa_K575027 Main Page]
  • [http://partsregistry.org/Part:BBa_K575029 BBa_K575029 Main Page]
  • [http://partsregistry.org/Part:BBa_K575030 BBa_K575030 Main Page]
  • [http://partsregistry.org/Part:BBa_K575031 BBa_K575031 Main Page]
  • [http://partsregistry.org/Part:BBa_K575035 BBa_K575035 Main Page]
  • [http://partsregistry.org/Part:BBa_K575038 BBa_K575038 Main Page]
  • [http://partsregistry.org/Part:BBa_K575039 BBa_K575039 Main Page]
  • [http://partsregistry.org/Part:BBa_K575040 BBa_K575040 Main Page]
  • [http://partsregistry.org/Part:BBa_K575041 BBa_K575041 Main Page]
  • [http://partsregistry.org/Part:BBa_K575044 BBa_K575044 Main Page]
  • [http://partsregistry.org/Part:BBa_K575045 BBa_K575045 Main Page]


Sponsor northwestern.jpg Sponsor weinberg.jpg Sponsor mccormick.jpg
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