Thursday, September 22

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Steve, Dr Liz present

We picked single colonies from the pET-Taq (4A4) in the 10-alpha strain of E. Coli growing on one of the two plates Dr Burkett brought for us. Made 5 samples for incubation in the 37° shaker:

Each of the first 4 tubes contained 3ml of the liquid growth medium, while the fifth tube only had 1.5ml. We lightly touched 4 separate colonies, putting those into the first 4 tubes. We dropped the tip used to pluck each cell sample into its respective tube. For the 5th tube we again touched the same colony that we touched for Tube 4. We are curious to see