qPCR curves

Probability data for the 783 fingers that bind to GNN triplets. Note the high probability of leucine at position 4 and arginine at position 6.

Probability data for the 128 fingers that bind to TNN triplets. Note the high probability of leucine at position 4.

Probability data for the 16 fingers that bind to CNN triplets. There may not be enough data to consider this information statistically significant

Probability data for the 29 fingers that bind to ANN triplets. There may not be enough data to consider this information statistically significant

Probability data for the 298 fingers that bind to NGN triplets. The position 4 leucine motif remains. There is also a high probability (> 0.5) of a histidine at position 3 and an arginine at position 6.

Probability data for the 177 fingers that bind to NTN triplets. The position 4 leucine motif remains.

Sequencing results of Library transformation

Error rates

Still being updated

• Good: 57.8% (22/38)
• Single SNP: 5.3% (2/38)
• Multiple SNPs: 18.4% (7/38)
• Frame shift: 18.4% (7/38)

Distributions

Still being updated Each of the sequenced zinc fingers have original F1 fingers.