Team:Caltech/Recipes

Agar/LB plate:

1) Fill two bottles with 500 g of nanopure water, 10 g of Tryptone, 10 g of NaCl, 5 g of yeast extract, and 15 g of Agar.

50% Glycerol Stock:
1) Pour 50 mL of Glycerol and 50 mL of pure water into a graduated cylinder.
2) Cover with parafilm and turn over a couple of times to mix.
3) Use the Nalgene vacuum to sterilize the stock.
4) Use the bunsen burner to keep air sterile and close the cap.

Enrichment Minimal Media

• 1.0712 g of K2 HPO4
• 0.5239 g of KH2 PO4
• 79 mL of Phosphate solution
• 100 mL of Salt Solution I
• 100 mL of Salt Solution II
• 10 mL of Wolfe's Vitamin Solution
• 1 mL of SL-10 Trace Element Solution.

and then add 5 mg of whatever chemical used in two flasks, one with vitamins and one without.